Ezrin tunes the magnitude of humoral immunity.
نویسندگان
چکیده
Ezrin is a member of the ezrin-radixin-moesin family of membrane-actin cytoskeleton cross-linkers that participate in a variety of cellular processes. In B cells, phosphorylation of ezrin at different sites regulates multiple processes, such as lipid raft coalescence, BCR diffusion, microclustering, and endosomal JNK activation. In this study, we generated mice with conditional deletion of ezrin in the B cell lineage to investigate the physiological significance of ezrin's function in Ag receptor-mediated B cell activation and humoral immunity. B cell development, as well as the proportion and numbers of major B cell subsets in peripheral lymphoid organs, was unaffected by the loss of ezrin. Using superresolution imaging methods, we show that, in the absence of ezrin, BCRs respond to Ag binding by accumulating into larger and more stable signaling microclusters. Loss of ezrin led to delayed BCR capping and accelerated lipid raft coalescence. Although proximal signaling proteins showed stronger activation in the absence of ezrin, components of the distal BCR signaling pathways displayed distinct effects. Ezrin deficiency resulted in increased B cell proliferation and differentiation into Ab-secreting cells ex vivo and stronger T cell-independent and -dependent responses to Ag in vivo. Overall, our data demonstrate that ezrin regulates amplification of BCR signals and tunes the strength of B cell activation and humoral immunity.
منابع مشابه
Humoral Immunity in Two Groups of Children Induced by Two Supplemental Doses of Oral Polio Vaccine, and in the Umbilical Cord Blood
متن کامل
Extract and leaf powder effect of Artemisia annua on performance, cellular and humoral immunity in broilers
The effects of methanolic extract and leaf powder of Artemisia annua were studied on performance, cellular and humoral immunity in 240 Cobb broiler chicks in a completely randomized design. Control group did not receive any treatment. The chicks in the second and third groups were fed with feed which contained 2000 and 4000 ppm plant extract in diet and in the groups 4, 5 and 6 chicks received ...
متن کاملHumoral immunity state in survivors of pediatric acute lympho-blastic leukemia
Background and Objective: Since there are very few studies on the immunodeficiency state of the Iranian survivors of pediatric acute lymphoblastic leukemia (ALL), we conducted this study to determine the prevalence of humoral defects in children with ALL at least one year after completion of chemotherapy. Methods: In this study, antibody titers for mumps, rubeola, rubella, tetanus and diphth...
متن کاملThe Effect of Oral Consumption of Guggul (Commiphora Mukul) Resin on Performance and Humoral Immunity Response of Broilers
This experiment was conducted to determine the effects of the oral consumption of guggulplant resin on the performance and humoral immunity response of broiler chicks. For doing this experiment, 320 one-day old chicks of the Cobb 500 strains, in a Completely randomized design with 4 treatments and 4 replicates were used. Experimental diets include: control diet (without the guggul), control die...
متن کاملThe Effect of Oral Consumption of Guggul (Commiphora Mukul) Resin on Performance and Humoral Immunity Response of Broilers
This experiment was conducted to determine the effects of the oral consumption of guggulplant resin on the performance and humoral immunity response of broiler chicks. For doing this experiment, 320 one-day old chicks of the Cobb 500 strains, in a Completely randomized design with 4 treatments and 4 replicates were used. Experimental diets include: control diet (without the guggul), control die...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of immunology
دوره 191 8 شماره
صفحات -
تاریخ انتشار 2013